Physiologic and Transcriptomic Insights into the High Alkali Response of Dunaliella salina

The halophilic unicellular green alga Dunaliella salina is characterized as the distinct tolerance to high salt in field. Normally organism concurrently subjected with and alkali stress, but feature of D. response stress was not investigated before. In this study, we used pH simulate found also showed even grown under at 11.0, cell density chlorophyll biosynthesis. High-throughput transcriptome analysis revealed dramatically different expression genes associated nitrogen metabolism, lipid β-carotene biosynthesis after treatment. Furthermore, physiologic that treatment induced activity nitrate reductase (NR), which generated nitric oxide (NO) messenger, ultimately accumulation Suppressing NR or NO generation reduced high-alkali-induced biosynthesis, well attenuated stress. Thus, our transcriptomic data reveal novel function NR-dependent signal during adaptation through inducing